IKAN TRANSGENIK PDF

IDENTIFIKASI IKAN CUPANG (Betta imbelis) TRANSGENIK FOUNDER MEMBAWA GEN PENYANDI HORMON PERTUMBUHAN; Identification of Transgenic. Ikan transgenik F-0 dibuat dengan menggunakan metode transfeksi. Identifikasi dilakukan menggunakan metode RT-PCR. RNA total. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are.

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Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi. Dengan demikian, metode krt-PCR adalah efektif untuk jkan sigositas secara cepat dan tepat pada ikan transgenik.

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Teknik ini dapat berguna dalam program produksi ikan transgenik secara massal dan dalam percobaan dimana faktor sigositas memberikan pengaruh nyata. Tingkat ekspresi transgen yang tinggi pada jaringan ikan F2 yang diperiksa telah diperoleh.

This method needs relatively large amounts of fish material and is time- and labor-intensive. Jurnal Akuakultur Indonesia Vol 2, No 1 In this experiment, we applied a quantitative real-time PCR qr-PCR method ijan analyze zygosity in a stable line of transgenic zebrafish Trznsgenik rerio carrying masu salmon, Oncorhynchus masou D6-desaturase-like gene. The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis.

JURNAl AQUACOASTMARINE

Highly unsaturated fatty acids HUFAespecially eicosapentaenoic acid EPA and docosahexaenoic acid DHA have long been recognized for its beneficial effect for human health and development. Produksi akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat peningkatan populasi manusia.

Jurnal Akuakultur Indonesia Vol 6, No 2 This Author published in this journals. Rapid method for identification of transgenic fish zygosity Jurnal Akuakultur Indonesia Vol 6, No 2 One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique.

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New approaches addressing this problem could be of great help for fish biotechnologists. The first F1 and second F2 generation was produced by crossing the transgenic founder F0 and F1, respectively, with wild-type fish. Pada penelitian ini, kami menggunakan metode PCR real-time kuantitatif krt-PCR untuk menganalisa sigositas pada satu strain ikan zebra Danio rerio transgenik yang membawa gen D6-desaturase-like dari ikan salmon masu, Oncorhynchus masou.

Transgenic individuals were identified by polymerase chain reaction PCR and their expression levels were analyzed by reverse transcription PCR. Expression of transgene in F2 generation was varied between strains regarding as the mosaics of F0 fish. Thus, we found that the qTR-PCR to be effective for a rapid and precise determination of zygosity in transgenic fish. Transgen telah ditransmisikan ke ikan generasi F2 dengan mengikuti pola segregasi Mendel.

Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur. This technique could be useful in the establishment of breeding programs for mass transgenic fish production and tfansgenik experiments in which zygosity effect could have a functional impact.

Teknik ini telah diaplikasikan pada spesies-spesies yang memiliki nilai ekonomis. Persilangan antara ikan transgenik tersebut dengan ikan normal menunjukkan transmisi transgen ke keturunannya mengikuti hukum segregasi Mendel. The intensity and capacity of production is expected to increase using biotechnology approach.

Pendekatan baru untuk mengatasi masalah tersebut akan memberikan manfaat besar kepada peneliti bioteknologi perikanan. Jurnal Akuakultur Indonesia Publisher: Now, a transgenic system to study the modification of fatty acid biosynthesis pathways in fish was established. Dengan demikian, sebuah sistem transgenik untuk memodifikasi biosistesa asam lemak pada ikan telah dikembangkan. This review describes various techniques of gene transfer, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture.

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Metode ini memerlukan ikan dalam jumlah yang banyak, dan juga waktu serta tenaga. Jurnal Akuakultur Indonesia Vol 6, No 1 Mating of those fish with wild-type had revealed transgene transmission to the offspring following expected Mendelian laws.

Recently, global food security has become a hot issue by the public in national as well as out of the country. Salah satu teknik modern yang diduga akan menjadi sarana yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are derived by mating the transgenic fish and wild-type counterpart.

Aquacultural output will need to be increased several fold in order to meet the rising demands for fish in coming years as the increasing of mankind population.

The transmission rate of transgene into F1 generation was 4. Data were analyzed using the comparative cycle threshold method. This technique has been applied to several commercially valuable species. Data dianalisis menggunakan metode pembandingan nilai cycle threshold.